The Effect of Antioxidants on Micropropagation of Avocado by Nodal Segments

Tissue culturing, which is an alternative method to clonal reproduction of avocado (Persea americana Mill.) has started become widespread in recent years. However, the browning tissue resulting from oxidation phenolic compounds one most important factors limiting success vitro avocados. Therefore, this study, effects different antioxidants (activated charcoal (AC), ascorbic acid (ASA), citric (CA) alone or combination with each other on and regeneration shoots, grown under conditions, were investigated. In MS (Murashige Skoog) nutrient medium 1 mg L-1 BAP, 0.1 GA3 3% sucrose was used all treatments. Moreover, combined concentrations media treatments except control. As a result research, lowest intensity highest survival rate values shown application 100 (ASA) 60 40 (CA). Furthermore, maximum shoot length (2.8 cm) leaf number (6.4) terms explants, leaves determined containing